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In some studies the annexin 1 blotted membrane was immersed in 10 ml of 0.5 N NaOH for 5 min at room temperature to strip off the annexin 1 blot followed by washing in distilled and deionized water and TBST buffer for 20 min.
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(PDF 284 KB) 12284_2014_28_MOESM7_ESM.pdf Additional file 7: Figure S5.: Northern blot analysis of cox1 and atp9.
Top row, FANCD2 blot of parental (K562) cells, two FANCD2 knockdown cell lines, and FANCD2 knockdown g2 with re-expressed FANCD2.
2. Blot the eggplants with paper towels and place, cut side down, on the baking sheets.
The following parameters were set up during blotting: blot force 8, blot time 8 s, temperature 25 °C, and humidity 100%.
3. Blot S., Vandewoude K., Colardyn F. Nosocomial bacteremia involving Acinetobacter baumannii in critically ill patients: a matched cohort study.
For the p21 blot, 60 mg of total protein was subjected SDS-PAGE and western blotting, whereas 25 µg was used for all other blots.
A Femto detection kit from Pierce (Rockford, IL) was used for film development of BMI1 blot.
There was no change seen with HGF stimulation (Fig. 4A, DRP-1 blot).
In the Chinese study where decreased cancer mortality was observed among individuals randomly assigned a combination of β-carotene, vitamin E and selenium, the mean baseline blood level of β-carotene was 5.9 6.8 μg dl−1 (Blot et al, 1993).
We performed the LC3 blot.
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