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Time course: 20 line scans for pre-bleaching (0.8 s), 5 for bleaching (0.2 s), and 500 for post bleaching (20 s).
The susceptibility of Acroporidae (and especially Acropora spp) to A. planci [20], bleaching [59], and disease [17] is well documented.
So, this crude enzyme could be used in combining enzymatic degumming or bioscouring and H2O2 bleaching.
All showed that it was very suitable for a joint process of enzymatic degumming and H2O2 bleaching.
Create a cleaning solution by mixing 1 cup (240mL) chlorine bleach with 1 gallon of warm water (3.75 liters).
A master thinning solution (10X concentration) was prepared by dissolving 1 bleach tablet in 100 ml distilled water.
Our results demonstrated that all 3 bleaching methods efficiently bleached melanin and partially destroyed tissue antigen.
Soak these in 3 cups of a bleach solution (consisting of about 5 parts bleach to 1 part water).
Time course: 5 scans for pre-bleaching and 60 scans of bleaching every 10 seconds.
(3) Bleaching: oocytes were bleached in 10% hydrogen peroxide/67% methanol for 12 48 h at room temperature under fluorescent illumination.
The FRAP image acquisition was divided into three steps: (1) pre-bleach, (2) bleaching, and (3) post-bleach.
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