After incubation for 3 h at 37 °C/5% CO2, Na-F was detected in basolateral samples (50 µl) using a microplate reader (Mithras2 LB 943, Berthold Technologies GmbH, Zug, Switzerland; excitation 485 nm, emission 528 nm).
Fluorescent signals of the samples were measured at an excitation of 485 nm and an emission of 528 nm using a microplate reader (Mithras2 LB 943, Berthold Technologies GmbH, Zug, Switzerland).
After washing of unbound tracer, chemiluminescence was measured (AutoLumat LB 953 Berthold Technologies, Bad Wildbad, Germany).
Immediate analysis was conducted during a 20-min period using a luminometer (AutoLumat Plus LB 953; Berthold Technologies, Bad Wildbad, Germany).
The pre-ocular retention of the TET-LCNP was assessed using a noninvasive fluorescence imaging system (NightOWL II LB985, Berthold Technologies, Germany) [22].
The measurement of green fluorescence (oxidized DCFH) using a microplate fluorometer (LB 941, Berthold Technologies, Bad Wildbad, Germany) with fluorescence intensity (excitation, 488 nm; emission, 530 nm).
The variation in the optical density of these solutions (absorbance at 620 nm) against time was measured at 37°C for 24 h with a microplate multimode reader (Mithras LB 940, Berthold Technologies, Germany).
The production of 1O2 was monitored by its dimol light emission or, indirectly, by the light emission generated by its reaction with melatonin using a plate luminometer (Centro Microplate Luminometer LB960, Berthold Technologies, Oak Ridge, TN, USA).
The intensity of fluorescence was measured with a fluorescence multi-well plate reader LB 941 (Berthold, Bad Wildbad, Germany) with fluorescence intensity (green: excitation 488 nm, emission 530 nm; red: excitation 535 nm, emission 605 nm).
Luciferase intensities were measured in a Centro Luminometer LB 960 (Berthold).
The reaction results are obtained by reading the optical density on a multi-plate reader Mithras LB 940 (Berthold, Germany).
