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The pseudotype virus was generated by co-transfecting 293T cells with two plasmids pcDNA-Sopt9 and pNL4-3Luc+Env−Vpr− carrying the optimized S gene and a human immunodeficiency virus type 1 backbone, respectively, as we previously described [14].
The molecule was generated by computer-aided fold analysis of the Bet v 1 backbone followed by site-directed mutagenesis.
In 2012, Bucardo et al. reported finding a vaccine-derived nonstructural protein 2 (NSP2) gene in 2 wild-type RVA strains with a G1P[8] genogroup 1 backbone (6 ).
Since membrane constituents such as phospholipids (PL) are easily detected in NMR-spectra, it was assumed that generic phospholipid signals (such as signals 18 and 20 in Figure 1; backbone signal for non-lyso-PL and lyso-PL) could be used as an internal measure for the amount of membrane and thus for the number of cells.
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The three facilities are scheduled to be fully operational on June 30, coinciding with Qwest's completion of its nationwide fiber-based T-1 backbone.
Citrate synthase (GltA) converted excess acetyl-CoA and oxaloacetate to citrate and was over-expressed by pJNU-4 (pCDFDuet-1 backbone).
Hughes To Use Level 3 Backbone For Spaceway.
This is also true for the DB18C6 backbone atoms.
Branched copolymers were obtained by grafting polyacrylamide (PAA) chains onto dextran (D70) backbone [26].
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