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In stage 1, aggregates of pre-nucleation clusters are in equilibrium with ions in solution.
cRILEM: Type 1 aggregates from masonry rubble, Type 2 aggregates from concrete rubble; Type 3 mixture of natural (min 80%%) and recycled (max 20%%) aggregate.
RILEM (1994): Type 1 aggregates from masonry rubble, Type 2 aggregates from concrete rubble; Type 3 mixture of natural (min 80%%) and recycled (max 20%%) aggregate.
Tissue sections with no lymphocyte aggregates were graded as 0. Grade 2 and grade 3 aggregates were termed large lymphocyte aggregates, while grade 1 aggregates were termed small lymphocyte aggregates.
Iloprost did not affect optical platelet counts in samples without aggregates or with score 1 aggregates.
We used the technique of multicolor single-molecule, super-resolution fluorescence imaging to characterize the growth of huntingtin exon 1 aggregates.
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As we observed using the Gallios flow cytometer (Beckman Coulter), at 488 nm excitation, valinomycin-treated cells (JC-1 aggregates negative) were poorly separated from the control cells (JC-1-aggregates positive) in bivariate plots of uncompensated data, and the 525/50-nm and 585/42-nm signals were strongly correlated (R=0.88).
At the hyperpolarized membrane potential (−140 mV) the red emission of the JC-1 aggregates shifts towards 590 nm.
The apoptotic cells contain somewhat fewer JC-1 aggregates and more JC-1 monomers and their colour shifts from orange to green.
Photomultiplier settings were adjusted to detect green flurorescence of JC-1 monomers on FL-1 detector and the red fluorescence of JC-1 aggregates on the FL2 detector.
The cells were washed with phosphate-buffered saline, and mitochondrial JC-1 aggregates were measured with a fluorescent plate reader (excitation 530 nm, emission 590 nm).
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