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Tier 1 (abridged) consultations involve questions not fully addressed by existing resources, but not so complex or broad in scope or impact that they require the input of the full Committee.
Reviewer #1 (abridged): In their appeal Dr. Richard and co-authors discuss two of the points I raised when re-reviewing the manuscript.
DOI: http://dx.doi.org/10.7554/eLife.06234.022 [Editors' note: the author responses to the re-review follow.] Reviewer #1 (abridged): As I wrote previously, I think the Richard group has "skipped the step, or are avoiding the issue, of establishing that the RGG box is important.
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The PCR product was reamplified with a primer pair of a nested gene-specific reverse primer (5'-atcatctggcttttgccatc-3' nucleotide positions 533 to 552, R552) and abridged universal anchor primer, resulting in the RACE product.
PCR amplification was accomplished by using Taq DNA polymerase with a primer pair of a nested gene-specific reverse primer (5'-tttggcacagttctcattgg-3': nucleotide positions 581 to 600, R600) and abridged anchor primer.
By "pruning" satellites in Text 1, we can produce an abridged version of this factorial explanation; for example: Text 2: Abridged version of Text 1, Physical Weathering.
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CEO of Professional Science Editing for Scientists @ prosciediting.com