Exact(47)
BAEC were then incubated for 0.5 hr with 1 µM LysoTracker Red (Invitrogen) and rinsed several times with PBS.
We next reduced the exposure time to either 0.5 hr or 1 hr and again observed nuclear retention of AR (Supplementary Fig. S3A).
For some experiments, at end of the 6 hr infection period, cells were washed and then stimulated with IFNγ (50 ng/mL for 0.5 hr at 37°C in 5% CO2), followed by whole cell protein extracts for immunoblotting.
Dark-reared p30 Q344ter mice in rho+/− or rho−/− background and their respective transgene-negative littermates were either subjected to light-exposure by dilating their pupils and exposed to 3000 lux for 0.5 hr or kept in the dark.
Plasma vWF transiently increased 0.5 hr after exposure.
All the larvae were collected between 0 and 0.5 hr after hatching.
Similar(13)
The model has three parameters, two of which can be estimated directly from the data: the exponential growth rate μ ~ 0.8 hr-1 and the bottleneck protein exponential increase rate ν ~ 0.5 hr-1.
Indeed, the '0.5 hr' network still satisfies all the predictions of BQS and strongly resembles the '0 hr' network.
DOI: http://dx.doi.org/10.7554/eLife.02863.034 Since a full eukaryotic transcriptional response typically requires >1 hr, we expect the '0.5 hr' network to be similar to the '0 hr' one.
For calculations involving animals aged 24 hr post mid-L4, indices were derived from the 0.5-hr time point of the time course in Figure 3 figure supplement 1, for consistency with Fox et al. (2011).
with overall survival outcomes by treatment arm and found that in the D+P group, a Pr(Decr-pPDGFR) >0.5 was associated with a median overall survival of 20 months (95% CI: 13.7 to 30+) vs >30 months (median, not reached) when Pr(Decr-pPDGFR) was ⩽0.5 (HR is 2.5 with 95% CI: 1.3 5.1; P=0.04 in log-rank test).
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