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And the insert can be removed for cleaning.
The nucleotide sequence of the insert was confirmed by sequencing.
(b) Introduction of the insert into the bracket's slot.
The corresponding athletes are identified in the insert.
Introduce the insert into the bracket's slot (Figure 11b).
This is highlighted in the insert in Fig. 4.
Showing when the insert came out.
For ALF63+5 the insert was 5'-CTCGA-3', for ALF63+10 the insert was (5'-CTCGAGTCGC-3', and for ALF63+30, the insert was 5'-GACGTCGCATACTCGAGGCCGCCATTACCT-3'.
The plasmids were verified by sequencing across the insert.
Interestingly, the insert orientation differed between the mutants.
The identity of the insert was confirmed by sequencing.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com