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The hid -lacZ reporter was found to respond in GMR-Dp53 eye discs and was utilized in this study as the hid reporter.
It is possible that the hid pathway is also mediated by Eiger.
Thus, a strong tTA expression seems to be important to start the hid Ala 5 expression early enough to cause complete embryonic lethality.
Comparable experiments using the hid 3′UTR reporter in S2 cells showed an unanticipated upregulation by both miR-6 and miR-11.
However, a recent report has provided independent evidence that miR-11 can function via smaller fragments of the hid 3′UTR containing the predicted sites.
The 30kb genomic DNA upstream of the hid start site was cloned in three parts with approximately 10kb each and a small region of overlap around 100 200bp.
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