Nor did the expression of constitutively active TCF (VP16-TCF) (Wong and Gumbiner, 2003) affect Nur77 expression induced by hypoxia.
In this case one TCF family member (TCF-3) inhibits keratinocyte differentiation and another (LEF-1) stimulates it.
The key effector of the Wnt signaling pathway is the bipartite transcription factor β-cat/TCF, formed by free β-cat and a member of the TCF family [TCF-1/TCF7, LEF-1, TCF-3/TCF7L1 and TCF-4/TCF7L2].
TOPglow features the gene for firefly luciferase, under control of a TCF promoter; FOPglow features the same reporter gene, but downstream of a mutated TCF-binding site.
Using a TCF reporter assay, we found that these FHC epithelial cells have no detectable TCF-mediated transcription.
Officials from JPMorgan and Capital One declined to comment; a TCF spokeswoman did not return a phone call seeking comment.
The crisis will follow a decline in the Marcellus shale which, although originally claimed to contain 410 tcf of gas reserves, in fact likely has just 50 tcf of technically recoverable reserves.
The US Energy Information Administration EIAA) has estimated that the UK has 26 trillion cubic feet (tcf) of technically recoverable shale gas.
The EIA can be wrong: it has downgraded its estimates for Chaco-Paraná, another Argentine basin, from 164 TCF to 3 TCF.
But in 2011, the EIA also posited that Poland possessed "some of the most favourable shale gas resources in Europe" which it estimated at 187 tcf.
Those in Capital One could be worth $241 million to $393 million, while those in TCF could fetch more than $12 million.
