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Later, TBP and TBP-associated factor 1 (TAF1) were revealed in mouse zygotes [ 30].
The ΔΔ CT method was used to quantify the mRNA using TBP (human) compared with Tbp (mouse) as housekeeping genes (TBP/ Tbp encodes TATA-box-binding protein).
TFIID is a multisubunit complex comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs).
These results demonstrate that the specific loss of TBP protein is the reason for the observed upregulation of the tbp promoter in TBP MO-injected embryos.
After the reaction, TBP ubiquitination was analyzed through western blot using an anti-TBP antibody.
After the reaction, TBP ubiquitination was analyzed through western blot using the anti-TBP antibody.
Consequently, NC2-induced lateral mobility of TBP was proposed to explain how NC2, by sliding TBP, could lead to TBP repositioning from/towards the promoter sites.
TBP was first suggested by Fishbein and Ajzen [ 16] and TIB is an extension of TBP.
TBP of compared to TBP of is calculated as (20).
After PVP was dissolved into the TBP.
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The TBP-specific MO (TBP MO) used was described previously (Müller et al, 2001).
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