Membranes were washed in TBST for 15 min, stripped in stripping buffer (Boston Bioproducts, BP-96) at 65°C for 30 min, followed by a stringent wash in TBST.
To normalise for protein content, the blots were stripped in stripping buffer (62.5 m M Tris-HCl, pH 6.7, 2% SDS, 100 m M β-mercaptoethanol) and stained with anti-actin antibody (Chemicon).
Membranes were then stripped in a stripping solution (62.5 mM Tris-HCl, 2% SDS, pH 6.7, and 100 mM β-mercaptoethanol) for 30 min with gentle rocking at 65°C.
That immunity was stripped in the security deal.
Murray, a fourth-year starter, was stripped in the pocket and fumbled the ball away.
But as so often happens these days, the provision was stripped in the Senate.
What he really stripped, in the end, was his audience's imagination.
Line that you have stripped in around your feet speeds back through the guides of the rod.
Stoudemire had 18 points but was stripped in the lane by Marcus Camby with the Knicks down by 97-95.
But the smooth limestone shaft is severe, almost modernistic, looking as if it had been stripped in a renovation campaign.
She then contacted him on Skype, stripped in front of him and persuaded him to perform a sex act.
