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We thank Roy Parker for RFP DCP2-RFP plasmid.
The primers used for RFP PCR were: RFP-f, 5′-GCATGGAGGGCTCCGTGAACG-3′ and RFP-r, 5′-GGTGTAGTCCTCGTTGTGGG-3′.
In contrast, nearly all untreated cells express RFP after identical Adv-RFP infection.
Intranasal instillation of mNPS loaded with dsDNA encoding RFP also resulted in expression of the RFP in multiple brain regions.
In this way, we created WT::RFP, A4V::RFP and G37R::RFP constructs along with a vector to express RFP alone.
RFP-iPSC-MSCs stably expressed high levels of RFP.
View the full RFP here.
That's basically what a personal RFP is.
(b) Percentage of RFP expressing nuclei as in (a) expressing YFP and RFP signal.
Send the updated RFP to potential vendors.
The PGIF RFP can be downloaded here.
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