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From a known amount of PHA in the biomass, the percent PHA recovered was calculated based on the purity of the total mass of the sample recovered at the end of the separation process.
PHA blasts were generated by stimulating 106/ml PBMC with 250 ng/ml PHA in T cell media supplemented with 50IU IL-2/ml.
Finally, applications of PHA in medicine are discussed.
3 Calculated as PHA in g L-1 divided by total fermentation time.
In this assay, isolated lymphocytes were stimulated by adding the phytohemagglutinin (PHA) in culture.
It corresponds to a typical "Preliminary PHA" in terms of the United States.
Similar(11)
Because we measured more mcl-PHA in Δ phaZ than its parent strain KT2440, we conclude that PHA depolymerase does not impose a negative effect on the PHA synthesis machinery.
Control of the monomeric composition of MCL-PHA in a fed-batch fermentation using a β-oxidation inhibitor is novel and challenging.
The objective of this study was to develop a methodology for controlling the monomeric composition of MCL-PHA in efficient fed-batch fermentations.
However, the role of PhaZ proteins in the synthesis of mcl-PHA in P. putida strains is not well understood.
The chimeric protein was localized to the Golgi compartment, was well expressed, and conferred resistance to L-PHA in stable CHO transfectant populations.
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