Two independently transformed lines were used for PCN gain of function analysis (35S::PCN-miRNAd 2-1 and 35S::PCN-miRNAd 2-3).
Two independently transformed lines were used for PCN knock down analysis (35S::miRNA-PCN 6-1 and 35S::miRNA-PCN 6-4).
To determine the duration of enhanced protection induced by the PCN treatment, PBS- and PCN-treated mice were challenged with influenza virus 3 to 35 days after completion of the PCN treatment.
To examine the function of PCN, we created transgenics expressing either an artificial miRNA targeting PCN transcripts (35S::miRNA-PCN) or a PCN cDNA with the endogenous miRNA recognition site mutated (35S::PCN-miRNAd).
Current recommendations for GBS prophylaxis which use PCN G should be modified pending future studies of neonatal PCN concentrations.
The PCN reduced as the molecular weight increased, and thus the PCN negatively correlated to the molecular mass of plasmids (Figure 1).
Of note, the largest plasmid pBMB400 had the lowest PCN (1.38) and the smallest plasmid pBMB2062 had the highest PCN (172).
Topic areas include: usual care for people with type-2 diabetes; existing PCN diabetes, depression and lifestyle programming and organisational factors and strategies related to PCN patient care.
