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RAW264.7 was incubated with tetramethyl rhodamine labeled biologically active MIF (MIF-TRITC) to visualize the internalization of MIF by using confocal laser scanning microscopy.
Inhibition of MIF and MIF-induced MMP-2 could be potential new therapeutic avenues for RA.
Here we tested whether exogenous MIF would induce MIF secretion.
In presence of doxycycline, MIF shRNA elicited a 90% decrease in MIF mRNA expression.
Conversely, MIF anti-sense treatment decreased both the intracellular MIF content and the amount of secreted MIF, suggesting that MIF anti-sense treatment interfered with MIF synthesis.
To elucidate the role of MIF in MMP-2 production, we produced zymosan-induced arthritis (ZIA) in MIF gene-deficient and wild-type mice.
Since macrophage migration inhibitory factor (MIF) is important for regulating innate immunity in bacterial and parasitic infections, circulating MIF and peripheral blood mononuclear cell (PBMC) MIF transcripts were investigated in children with acute falciparum malaria.
Circulating MIF represents a considerable source of MIF [9] and presents a possible confound when examining organ levels of MIF.
"Is MIF high-end?
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Treatment with HA, anti-MIF antibody and MIF anti-sense reduced HT-1376 cell proliferation, MIF protein secretion, MIF gene expression and secreted inflammatory cytokines.
pCMV-3xFLAG-MIF significantly increased MIF protein expression (Fig. 1A, left panel).
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