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The resulting three MFI values are Particle-MFI, Colloid-MFI, and Organic-MFI, and presented schematically in a triangle manner.
Abnormal MFI is defined as MFI < 2.6.
Abnormal MFI is defined as MFI < 2.6 for small vessels (diameter < 20 μm).
a Represents two subgroups, separated by microvascular blood flow (MFI) < 2.6 versus MFI ≥ 2.6.
Mean fluorescence intensity Ratio (MFI) was calculated as MFI of sample/MFI of isotype control.
Fold change was calculated by dividing the MFI of the treated sample (MFI treated) from that of the control sample (MFI untreated), i.e., fold change percent % = (MFI treated/MFI untreated) ×100[22].
The nuclei which did not stain for NFATc2 (MFI = 11.9) had the same MFI as the nuclei from unstimulated Th cells (MFI = 11.2).
MFI values were adjusted by subtracting the MFI of cells negative for expression.
The cutoff value was 3458 MFI with signals ranging between 2997 and 8525 MFI (mean=5870).
The geometric mean fluorescence modulation ratio is calculated as: (MFI with modulator - cold control MFI /(MFI with diluent control - cold control MFI) [ 53, 54].
Percent change in MFI was calculated as the difference between the MFI values after and before transfusion divided by MFI values before transfusion.
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