In the meanwhile, compared to BsNudF, EcNudF has a much poorer substrate specificity towards IPP, making IPP can't be efficiently hydrolyzed.
Throughput = 100 - left[ CPI times IPP)_{LR}/ CPI times IPP)_{Tomasulo}right] times 100 = 95 (3).
Hence, Eq. (1) becomes, Throughput = 100 - left[ CPI times IPP)_{LR}/ CPI times IPP)_{In-order}right] times 100 = 98 (2).
Anti-fibrillarin was not detected by IPP in our previous report, although the IPP method used was not optimized for the detection of this antibody [ 14].
IPP isomerase catalyzes the conversion of relatively unreactive IPP to electrophile dimethylallyl pyrophosphate (DMAPP).
Further, MEP is transformed to IPP and DMAPP, condensation of two molecules of IPP with DMAPP, leading to FPP, step catalyzed by FPS.
This uneven implementation of IPP within our study is consistent with the mixed results of IPP found in the literature [ 23, 29].
This study goes some of the way towards unravelling the complexity of IPP in rural contexts, highlighting the strong motivating factors that drive IPP.
The binding affinities of IPP to BTN3A1 and the Vγ9Vδ2 TCR interaction with BTN3A1-IPP are summarised in Table 1.
Therefore, even with supportive IPP in place, implementation could still fail if the intended users do not feel the effects of these IPP as encouraging implementation.
As we expected, IPP indeed induced significantly expansion of purified γδ T cells when we repeated the H-TdR incorporation assay using IPP as positive control.
