Emory University in Atlanta, Georgia, for example, has announced plans to establish a laboratory at the ICGEB in New Delhi, staffed by scientists from its Atlanta campus.
Cloning of GFP gene into pBAD33 was done by digesting pET14b-GFP (obtained from ICGEB, India) with enzymes Xba I and Hind III and ligating it into plasmid pBAD33 (which does not contain any ribosome binding site).
Frozen samples were transported to the International Centre for Genetic Engineering and Biotechnology (ICGEB) in New Delhi, and kept in liquid nitrogen until thawed for cytoadherence assays.
The authors are grateful to Professor Virander S. Chauhan, Director (ICGEB) for providing all the necessary facilities for conducting the experiments and critically reviewing the manuscript.
We thank DBT-supported Tuberculosis Aerosol Challenge Facility (DBT-TACF; BSL-3 containment) staff at ICGEB, New Delhi, India for their kind help.
All animal experiments were conducted in accordance with guidelines approved by the Institutional Animals Ethics Committee meeting held on 22nd November 2007 at ICGEB (approval ID; ICGEB/IAEC/IMM-13/2007), New Delhi, India and Department of Biotechnology (DBT) guidelines, Government of India.
We acknowledge H. K. Prasad, AIIMS, New Delhi, India; Amit Singh, ICGEB, New Delhi, India; Vivek Rao, NIMR, London, UK and Priyanka Chauhan, UDSC, New Delhi, India for critical reading of the manuscript and valuable suggestions.
C57BL/6 and PD-1 deficient mice (obtained from Dr. Tasuku Honjo, Yoshida-konoe, Sakyo-ku, Kyoto University, Japan, via Dr. Megan Sikes, Transplantation Biology Research Center, Harvard Medical School, Boston, MA, USA) on a C57BL/6 background were bred in our specific pathogen-free animal facility at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
Funding for open access charge and for the services of a scientific writer/editor: ICGEB.
Prof. Sandor Pongor, International Centre for Genetic Engineering and biotechnology (ICGEB), Italy Accepted.
