The plasma disappearance rate of ICG (ICG-PDR) was calculated automatically by the time course of the blood ICG concentration (normal value: ICG-PDR ≥ 16%/minute).
Figure 3B represents the fluorescence intensity per unit of ICG slope at typical ICG concentrations for three lipid-bound ICG samples (equivalent to molar ratios of 100 1, 250 1, and 350 1) and a soluble ICG control.
Therefore, the ICG plasma disappearance rate (ICG-PDR) is used as a dynamic liver function test.
Serum was collected before and at 5, 10 and 15 minutes after the ICG injection to determine the ICG retention rate at 15 minutes (ICG R15).
To evaluate the interactions between ICG and lipid, we incubated empty (no ICG) liposomes with varying concentrations of ICG.
This result suggests that encapsulation of the ICG within PFC/ICG nanoemulsions could successfully protect the ICG fluorescence signals against external environments.
d Fluorescence spectra of ICG and rNGO-PEG/ICG solutions with the same concentration of ICG.
However, The ICG in PFC/ICG nanomulsions maintains the chemical stabilization and reduces the ICG molecular aggregates.
The quantity of ICG loaded into the PFC/ICG nanoemulsions was determined from the free ICG that was not incorporated into the PFC/ICG nanoemulsions.
With this lipid composition, we achieved complete incorporation of ICG by mixing ICG and lipids together in the organic phase, producing stable liposomal ICG particles.
ReprintsThe ICG may overdo the dangers.
