Exact(60)
On average at least 2 LUVs were bound to each GUV and were mobile on the GUV surface.
Nevertheless, introducing reasonable differences in osmotic pressure which results in GUV inflation or using a different lipid composition without cholesterol did not significantly affect the GUV deformation process and the formation of protocellular junctions (Supplementary Fig. S5).
Surprisingly, instead of bursting, the GUV membrane shrunk from the rim of the pore toward the opposite side of the GUV (Movie S7).
For GUV permeabilization assays, BCL2 family proteins were mixed in KHE buffer with FITC-4-kDa dextran, APC and GUV which had been doped with DiD.
What, guv?
Honest, guv.
No way, guv.
Take the gift, guv.
Search me, guv.
She called him Guv.
Not us, guv.
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