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Two human GBC cell sublines, GBC-SD and GBC-SD/M, which possess lower and higher metastatic potential, respectively [ 21], were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China).
KIAA0125 was upregulated in a highly metastatic GBC cell subline GBC-SD/M, in contrast to GBC-SD cell subline with a relative lower metastatic potential.
Despite the significantly better response rate in GBC, patients with GBC had a shorter median overall survival (7.2 months) than those with BDC (9.3 months; P=0.048).
We found KIAA0125 expression is incredibly elevated in a GBC cell subline GBC-SD/M with higher metastatic potentials as compared to the other subline GBC-SD cells [ 21].
Four human GBC cell lines (GBC-SD, SGC-996, NOZ and EH-GB2) were used in this study.
Furthermore, knockdown of VLDLR with siRNA can inhibit proliferation of GBC cells, whereas enforced VLDLR expression promotes GBC cell proliferation.
Atomic absorption spectrometry (AAS) with hydride generation system (GBC 3000) and background corrector (GBC 932 plus GBC scientific equipment's, VIC, Australia) was used in University of Kelaniya to detect arsenic.
The expression of Shepherdin in gallbladder carcinoma (GBC) cells was detected and its strong inhibitory effects against GBC growth were evaluated after AAV mediated gene transfer of Shepherdin into GBC cells and xenograft tumors.
Our data suggests that MIF is active in GBC and plays a pivotal role in cellular proliferation and invasiveness of GBC.
GBC are twice more common in females and expression of estrogen receptors in GBC has been shown.
Early diagnosis and treatment of GBC requires elucidation of molecular events associated with tumor progression and aggressiveness in GBC.
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