Sentence examples similar to * flp in from inspiring English sources

(A) SiRNA transfected Flp-In-293 cells were treated with tetracycline as indicated.

To this end, we generated Flp-In-293 cells to stably express wild-type or mutant Sec61β (Fig. S2F).

A CHO cell line stably expressing ROBO1 fused with an HA tag (ROBO1-HA) was generated using the Flp-In System (Life Technologies Japan Corp., Tokyo, Japan).

For flow cytometry, wild type or ROBO1-HA-expressing Flp-In-CHO cells were incubated with primary antibodies for 1 h at a concentration of 1 μg/mL.

(c) Dose-dependent reactivity of the anti-ROBO1 IgG by cell ELISA (filled circle, wild type CHO-cells; open circle, ROBO1-HA-expressing Flp-In-CHO cells).

The selected stable integrants were 100% homogeneous, an important attribute of the Flp-in system.

Constructs were cloned into vector pcDNA5/FRT (Invitrogen) and transfected into CHO Flp-In-cells (Invitrogen).

The Flp-In system provides an advantage over the transient transfection approach.

For flow cytometry, U2OS flp-in cells expressing various FLAG-tagged Notch polypeptides were trypsinized to create single cell suspensions.

The generation of HEK293-EGFR cells (HEK Flp-In-293 cells expressing ectopic EGFR) has been described [17].

Cell lines stably expressing MIP-1α constructs were generated using the Flp-In System (Invitrogen) according to the manufacturer's instructions.