However, in epidemiologically unrelated strains, these mutations accounted for EMB resistance in only around 30% EMB-resistant isolates [ 13, 18].
The measured EMB concentration in the nominal 200 μg L-1 EMB treatment was 99.5 ± 5.2 μg L-1 EMB.
Only one study has so far detected mutations in other emb (embA and embC) or other genes conferring resistance to EMB in epidemiologically unrelated EMB-resistant strains [ 13].
The levels of phosphorylated p70S6K (Fig. 6C) were significantly higher in the EMB plus rottlerin (EMB+R -treated group comparEMB+R -treatedB-treated group.
Sixteen of 51 mapped emb mutants examined were found to be disrupted in a known EMB gene.
The isolates were considered EMB resistant when bacterial growth occurred at a concentration of 2.5 μg EMB per ml.
At each concentration of EMB the relative average growth rate compared to the unexposed control (0 mg/L EMB) was calculated for days 7 9, 0 to 2 days EMB exposure.
Mutations in the embB gene and those within its conserved EMB resistance determining region (ERDR) in particular have repeatedly been associated with resistance to EMB in Mycobacterium tuberculosis.
