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(B) Quantitative RT-PCR analysis of the expression levels of miR-10a, the relative expression levels were analyzed using ΔCt values (ΔCt = CtmiR-10a − CtU6).

Figure S1 illustrates the ΔCT values (CTsample-CTmiR-145).

ΔCT and ΔΔCT were calculated using the following mathematical formula: ΔCT = CTsample − CTmiR-24, ΔΔCT = ΔCTcase − ΔCTcontrol.

For tissue samples, the relative amount of miR-24 to U6 was calculated as 2−ΔCt method, where ΔCt = (CtmiR-24 − CtU6).

The change for mRNA in HCC tissues relative to the matched normal liver tissues was calculated using the 2−ΔΔCt method, where ΔΔCt = ΔCt HCC/normal liver tissues and ΔCt = CtmiR-20b − CtGAPDH.

ΔΔCt= (CtmiR-21 CtmiR-16 CtmiR-21 CtmiR-16 CtmiR-21 CtmiR-16R-16)meen of the coNSCLC group.