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Of the 11 genes tested, 8 were found to be concordant in the RQ-RT-PCR validation (73% concordance rate).
WB and Biokit were concordant in 408 negative and 325 positive results for an overall concordance of 93.7%.
MAPK pathway mutations were 98.4% concordant in matched primaries and metastases (60/61; 95% CI for proportion of concordance, [91.2%, 100.0%]) (Table 2).
Thus, microbiological data were fully concordant in 78 cases (75%%).
Scintigraphy was concordant in 22 (96 %), n = 23.
Tc-99m sestamibi scintigraphy was concordant in 22 (95.7 %), n = 23.
Probability model proved to be concordant in 91% of the calibration set observations.
BRAF mutation status was concordant in 95.2% of the 62 paired samples.
Clinical and ultrasound evaluation were not concordant in 50% of patients, the former being more likely to miss hypovolemia.
169 patients also had replicate blood sampling; PCR/ESI-MS was concordant in 85% of cases, culture in 55%.
In the asymptomatic population, the results were concordant in 298/300 (99.3%), seven positive and 291 negative.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com