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Shigella dysenteriae CMCC 51252 and Shigella flexneri CMCC 51571 were purchased from the National Center for Medical Culture Collection (CMCC) (Beijing, China).
These derivatives were initially evaluated for their in vitro antimycobacterial activity against M. phlei CMCC 93201 and M. smegmatis CMCC 93202.
E. coli O111 (CMCC44151) was purchased from National Center for Medical Culture Collections (CMCC, Beijing, China).
The titers or capacities of antisera against rOmpF and bacteria (E. coli CVCC 1515, E. coli CICC 21530, S. dysenteriae CMCC 51252, S. flexneri CMCC 51571, S. enteritidis CVCC 3377, S. pullorum CVCC 503, P. aeruginosa CICC 21630, and P. aeruginosa CICC 10419) were measured by iELISA (Guan et al. 2015; Hu et al. 2010).
Additionally, the capacities for the anti-rOmpF sera binding to bacterial cells were performed among the strains of E. coli CVCC 1515, E. coli CICC 21530 (O157 H7), S. dysenteriae CMCC 51252, S. flexneri CMCC 51571, S. enteritidis CVCC 3377, S. pullorum CVCC 503, P. aeruginosa CICC 21630, and P. aeruginosa CICC 10419 in vitro.
All of the synthesized compounds were less active than balofloxacin against M. phlei CMCC 93201 and M. smegmatis CMCC 93202, but compounds 3g j (MIC: <0.5 8 μg/mL) were more potent than balofloxacin (MIC: 16 μg/mL) against MTB 09710.
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The methodology here described has been applied to evaluate the scalability of the CMCC-MED coupled model designed by the ANS Division of the CMCC.
CMcC: It's not brave on my part.
CMcC and LJM are joint guarantors.
CMcC was involved in the administrative support, data acquisition and final approval of the manuscript.
CMcC did the main data analysis with input from VHS, CM and PD.
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