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Dehydroascorbate was calculated by the difference between total ASC and reduced ASC.
During inflammasome activation, ASC molecules aggregate and form ASC specks (also known as ASC foci or pyroptosomes).
Microglia also release complexes of the protein ASC (a constituent of the inflammasome) called ASC specks.
We observed that secreted oligomerized ASC was phosphorylated and quercetin inhibited phosphorylated-ASC secretion (Fig. 4E G).
Having defined ASC phenotype, we then sought to assess rabbit ASC multilineage potentials.
The timing of ASC injection might be important; therefore, we divided ASC-injected mice into two groups: ASC pre and ASC post.
Furthermore, ASC phosphorylation is not required in NLRC4 inflammasome while NLRP3 and AIM2 inflammasomes require ASC phosphorylation22.
ASC dimers were detected in LPS + Nigericin stimulation, but the quercetin-treated cells had less ASC dimerization compared with controls (Fig. 4C,D).
(B) Percentage of cells containing ASC speck.
The white arrow represents ASC specks.
QUC reduced ASC speck formation and ASC oligomerization compared with controls.
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