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For AIF release, AIF (Santa Cruz Biotechnologies, sc-9416) was utilized instead of cytochrome c antibody.
PAD inhibition prevents association of PAD3 with AIF and AIF cleavage required for its translocation to the nucleus.
Importantly, AIF overexpression effectively reversed AIF knockdown-induced PTEN oxidation and Akt phosphorylation (Fig 3H).
As shown in Figure 4C, AIF siRNA markedly downregulated AIF content.
We therefore assessed whether there was any direct interaction of AIF with the cytoskeleton by AIF immunoprecipitation.
As shown in Figure 3B, 200 n M AIF siRNA significantly attenuated whole-cell AIF level as well as mitochondrial and nuclear AIF content.
Together, these results indicate that AA005 promote AIF nuclear translocation and trigger AIF-dependent cell death.
Cytoplasmic cytochrome c & AIF and also the nuclear translocation of AIF were analyzed by western blot.
Calpain cleavage of AIF could be associated with the release of AIF from the mitochondria in particular cases of PARP-1-dependent AIF cell death.
The adult harlequin (Hq) mouse has about 80% less brain AIF due to a proviral insertion in the AIF gene.
With the war over, the AIF was demobilised, and Mackay's appointment to the AIF was terminated on 4 April 1920.
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